Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy

verfasst von

Christoph Wetzel, Linda Jansen-Olliges, Marc Stadler, Frank Surup, Carsten Zeilinger, Bernhard Roth

Abstract

The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.

Organisationseinheit(en)

Hannoversches Zentrum für Optische Technologien (HOT)
Institut für Organische Chemie
Zentrum für Biomolekulare Wirkstoffe (BMWZ)
Angewandte Photonik - Optische Analytik, Sensorik und Spektroskopie
PhoenixD: Simulation, Fabrikation und Anwendung optischer Systeme

Externe Organisation(en)

Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
Technische Universität Braunschweig

Typ

Artikel

Journal

Biomedical optics express

Band

14

Seiten

4097-4111

Anzahl der Seiten

15

ISSN

2156-7085

Publikationsdatum

01.08.2023

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Atom- und Molekularphysik sowie Optik, Biotechnologie

Elektronische Version(en)

https://doi.org/10.1364/BOE.495685 (Zugang: Offen)