Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy

authored by

Christoph Wetzel, Linda Jansen-Olliges, Marc Stadler, Frank Surup, Carsten Zeilinger, Bernhard Roth

Abstract

The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.

Organisation(s)

Hannover Centre for Optical Technologies (HOT)
Institute of Organic Chemistry
Centre of Biomolecular Drug Research (BMWZ)
Photonics - Optical Analytics, Sensing and Spectroscopy
PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines

External Organisation(s)

Helmholtz Centre for Infection Research (HZI)
Technische Universität Braunschweig

Type

Article

Journal

Biomedical optics express

Volume

14

Pages

4097-4111

No. of pages

15

ISSN

2156-7085

Publication date

01.08.2023

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics, Biotechnology

Electronic version(s)

https://doi.org/10.1364/BOE.495685 (Access: Open)