A miniaturized test for stress biomarker detection by quantum dots on microarrays

authored by: Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, Carsten Zeilinger
Abstract: We aim to develop a benchtop imaging device to quantitatively measure stress biomarkers like HSP90 on a biochip. Measurement of HSP90 is based on the masking of fluorescence of Streptavidin conjugated Quantum dots (Sav-QDs) when HSP90 attaches to it. The higher the masking of fluorescence, the higher the HSP90 concentration. Measurement of fluorescence is done by a custom-built optical device. The goal in this work was to demonstrate a testing system which is handy to use in the field, cheaper and with a simplified readout system for the users. Sav-QDs are fixed on nitrocellulose coated glass slides (NC slides). A biotinylated antibody is attached to Sav-QD. When HSP90 interacts with the antibody it causes masking of fluorescence, which is dependent on the concentration of HSP90 in serum. The imaging device developed for measurement is based on a CMOS sensor. It uses narrow bandpass filters, optically eliminating fluorescence produced by the background. Analysis of the results shows that these agree well with those of standard laboratory equipment using a photomultiplier tube (PMT) scanner to detect HSP90 in the nanomolar range. Results from the work show that the used approach is promising for developing a multifunctional, robust, and point-of-care detection system for stress biomarkers.
Organisation(s): Hannover Centre for Optical Technologies (HOT)
PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines
Centre of Biomolecular Drug Research (BMWZ)
Type: Conference contribution
No. of pages: 9
Publication date: 27.03.2024
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Electronic, Optical and Magnetic Materials, Atomic and Molecular Physics, and Optics, Biomaterials, Radiology Nuclear Medicine and imaging
Electronic version(s): https://doi.org/10.1117/12.3002242 (Access: Closed)