Brillouin and Raman imaging for plant cell wall mechanics

authored by

Timm Landes, Inga Weisheit, Alphonse Mathew, Eren Alkanat, Miroslav Zabic, Thomas Debener, Dag Heinemann

Abstract

Plant cell walls are highly complex structures, which are adaptable to various environmental stresses. While many biochemical pathways have been discovered, cellular stress indicators are widely unknown. Here we demonstrate a label-free optical setup, that can map both mechanical properties and chemical composition in biological samples. We measured changes in mechanical stiffness and chemical response of rose leaf cells due to inoculation of the fungal disease Diplocarpon rosae. With this we are able to image fungal growth in a dynamical and high-resolution manner.

Organisation(s)

Phytophotonics
Abteilung Phytophotonik
Section Molecular Plant Breeding
PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines

Type

Conference contribution

Publication date

16.03.2023

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials, Atomic and Molecular Physics, and Optics, Radiology Nuclear Medicine and imaging, Biomaterials

Electronic version(s)

https://doi.org/10.1117/12.2649829 (Access: Closed)