Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

authored by

Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, Carsten Zeilinger

Abstract

We report on a simplified optical imager to detect the presence of a stress biomarker protein, namely the Heat shock protein 90 (Hsp90). The imager consists of two elements the optical unit and the sensor, which is a custom-made biochip. Measurement is based on the masking of the streptavidin conjugated quantum dot's (Sav-QDs) fluorescence when Hsp90 attaches to it via biotinylated antibodies (Ab). The masking effect was directly proportional to the Hsp90 concentration. The cost-efficient benchtop imager developed comprises a CMOS sensor, standard optical lenses, and a narrow bandpass filter for optically eliminating background fluorescence. This approach is promising for the realization of cheap, robust, and reliable point-of-care detection systems for various biomarker analyses.

Organisation(s)

Hannover Centre for Optical Technologies (HOT)
PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines
Centre of Biomolecular Drug Research (BMWZ)

Type

Article

Journal

Biomedical optics express

Volume

15

Pages

4147-4161

No. of pages

15

ISSN

2156-7085

Publication date

07.06.2024

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Atomic and Molecular Physics, and Optics

Electronic version(s)

https://doi.org/10.1364/BOE.527338 (Access: Open)